Journal: REPRODUCTION

Article Title: Dickkopf-1 secreted by decidual cells promotes trophoblast cell invasion during murine placentation

doi: 10.1530/rep-07-0191

Figure Lengend Snippet: Figure 1 Immunofluorescence of Dkk1 and b-catenin on day 8 in pregnant uterus, cultured EPC, and detection of Dkk1 secreted by decidual cells through ELISA. Green signals represent Dkk1 and b-catenin staining, and red signals indicate nuclear staining. Yellow color represents an overlap of green and red signals. BarZ50 mm. (A) Dkk1 expression on day 8 in pregnant uterus. (B, G, and H) b-Catenin expression on day 8 in pregnant uterus. (G) A higher magnification of the white circled area in (B). (H) A higher magnification of the purple circled area in (B). (C) Dkk1 expression in cultured EPC. (D) b-Catenin expression in cultured EPC. (E) Dkk1 expression in cultured decidual cells. (F) Negative control using preimmune goat IgG. (I) The standard curve was constructed by plotting the log of the Dkk1 concentrations versus the log of the OD. The score of Dkk1 was the average of results from three individual experiments.

Article Snippet: Culture supernatants from decidual cells were concentrated for 20 times using the Amicon Centricon filter device from Millipore Corporation (Billerica, MA, USA)after culture for24 h. ELISAwas performedusing theDuoSet ELISA Development System for mouse Dkk1 (R&D Systems) according to manufacturer’s protocol.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Negative Control, Construct

Journal: REPRODUCTION

Article Title: Dickkopf-1 secreted by decidual cells promotes trophoblast cell invasion during murine placentation

doi: 10.1530/rep-07-0191

Figure Lengend Snippet: Figure 2 The effects of Dkk1 on EPC attachment and outgrowth. (A) Effect of endogenous Dkk1 secreted by decidual cells. The efficiency of EPC attachment and outgrowth after being cultured alone, co-cultured with decidual cells or pretreated decidual cells with Dkk1 blocking antibody for 24 h beforeco-culture. (B) The effect ofrecombinant mouse Dkk1. The efficiency of EPC attachment and outgrowth after treatment with 200 ng/ml Dkk1. Results were expressed as meanGS.E.M. of three replicates. *, ** and ***indicate significant difference from the control (P!0.05, P!0.01, and P!0.001 respectively).

Article Snippet: Culture supernatants from decidual cells were concentrated for 20 times using the Amicon Centricon filter device from Millipore Corporation (Billerica, MA, USA)after culture for24 h. ELISAwas performedusing theDuoSet ELISA Development System for mouse Dkk1 (R&D Systems) according to manufacturer’s protocol.

Techniques: Cell Culture, Blocking Assay, Control

Journal: REPRODUCTION

Article Title: Dickkopf-1 secreted by decidual cells promotes trophoblast cell invasion during murine placentation

doi: 10.1530/rep-07-0191

Figure Lengend Snippet: Figure 3 The effect of antisense Dkk1 ODN on the migration of sTGCs toward decidual cells in vivo. (A–D) Detection for the inhibition efficiency of antisense Dkk1 ODN at mRNA and protein levels. (A) mRNA levels of Dkk1 were detected after treatment of decidual cells with 20 mM sense or antisense Dkk1 ODN. (B) Densitometric analysis of RT-PCR for Dkk1. Results are presented as meanGS.E.M. of three triplicates (**P!0.01). (C and D) Immunostaining of Dkk1 after treatment of mouse uterine epithelial cells with 20 mM antisense (C) or sense (D) Dkk1 ODN. (E) Implantation sites on day 8 after intrauterine injection of antisense or sense Dkk1 ODN on day 5 of gestation. (F) The number of implantation sites was counted on day 8 of gestation after injection. (G) and (H) H&E staining of paraffin sections of implantation sites after intrauterine injection. (G) Injection with antisense Dkk1 ODN. (H) Injection with sense Dkk1 ODN.

Article Snippet: Culture supernatants from decidual cells were concentrated for 20 times using the Amicon Centricon filter device from Millipore Corporation (Billerica, MA, USA)after culture for24 h. ELISAwas performedusing theDuoSet ELISA Development System for mouse Dkk1 (R&D Systems) according to manufacturer’s protocol.

Techniques: Migration, In Vivo, Inhibition, Reverse Transcription Polymerase Chain Reaction, Immunostaining, Injection, Staining

Journal: Nature communications

Article Title: Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancer.

doi: 10.1038/s41467-025-56420-w

Figure Lengend Snippet: Fig. 8 | DKK1 levels correlate with reduced therapeutic responses and cytotoxic NK cells. A Schematic representation of NK cell isolation from healthy donor human PBMCs and incubation with target cells in the presence of PBS or rhDKK1 for 4 h. B–D Analysis of percent specific killing measured by 7-AAD+ MDA-MB-231 (B, n = 7 NK donors), T47D (C, n = 3 NK donors), and K562 (D, n = 3 NK donors) target tumor cells after 4 h incubation with human NK cells in the presence of rhDKK1 or PBS as a control. E FACS analysis of NK cell activating receptors on human NK cells from different donors (n = 11) stimulated with rhDKK1 for 24 h or PBS. F Schematic representation of blood sample collection of advanced breast

Article Snippet: Serum or plasma levels of DKK1 frommice or patients were quantified using ELISA kits specific for DKK1 (Mouse Dkk1 Quantikine ELISA kit or Human Dkk1 Quantikine ELISA kit, R&D systems) as per the manufacturer’s protocols.

Techniques: Cell Isolation, Incubation, Control